Ordering For Automated DNA Sequencing Service
Order No./ Date:
Name of the scientist:
Email:
Institute:
Address:
Any preliminary identification information about the strain:
Whether the sample is infectious
Yes
No
More culture of microbes will be provided if more sample is required:
Yes
No
SPA Services
Total number of samples:
Total number of reactions:
Sample sent as:
--choose--
Plasmid DNA
Stab
PCR Products
Growth condition if sent as stab/ plate:
--choose--
Antibiotic used
Temperature
Size of the plasmid / vector DNA:
Information of the restriction site to release insert:
Approximate size of insert:
Total amount of DNA supplied:
µg as lyophilized/ in water
Type of DNA template:
--choose--
GC Rich
AT Rich
Secondary Structure
Long Repeats
Homopolymer Region
Others
Purification protocol used:
--choose--
Alkalyn lysis with PEG pptn
Column
Resin
Others (sepicify the column used for DNA purification)
Primer to be sequenced with:
--choose--
M13FP
RP
T7U
T3
SP6
SK
KS
Others
If primer supplied by scientist:
Yes
No
Purified primer supplied (PAGE/HPLC) Sequence of primer / length:
Quantity of primers supplied:
Annealing temperature used by scientist:
Primer walking services:
Initial primers to be sequenced with:
Sequencing to be done for:
--choose--
One strand
Both Strands
Primer Walking Services
Initial primers to be sequenced with:
Sequencing to be done for:
--choose--
One strand
Both Strands
Related Links
4
Auto DNA Sequencing
4
Bacterial Identification
4
DNA Fingerprinting
4
Gene Synthesis,Cloning
4
Cloning & Sequencing
4
Vivo Site Directed Mutagenesis
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