GeneOmbio Technologies provides preset as well as customized hands-on training to candidates from academic institutions as well as industry. The training module includes the basics of recombinant DNA technology (DNA extraction, PCR etc) and handling of laboratory strains of microbes. It also has advanced components such a automated DNA sequencing and application related to Real Time PCR. Further, the candidate is exposed to use of different bioinformatics tools and analysis skills that form an integral part of modern molecular biology. The details of such training are available on request. The company also provides customized training courses. This is required when candidates wish to focus intensely on few aspects of the training module in greater depth. However, this warrants interaction between the candidate / teacher to the candidate and geneOmbio management in order to design the customized module. For prospectus/ further information please contact our marketing team.

Module for Advanced Training in Molecular Biology

1. DNA extraction:
   1. DNA extraction from plant/bacterial sources.
   2. Spectrophotometric estimation of DNA; OD ratio - 260/280 nm.
   3. Agarose gel electrophoresis of DNA
   4. Restriction digestion with a hexamer restriction endonuclease; viewing the banding profile on an agarose gel.

2. Molecular cloning of insert and transformation of E. coli with a recombinant plasmid DNA:
   1. Competence of laboratory strain of E. coli by the Calcium chloride method.
   2. Shotgun cloning of restricted genomic DNA into linearized vector DNA molecule.
   3. Its transformation with a multi copy plasmid DNA harboring drug resistance selectable genetic marker.
   4. Selection of recombinants and its purification.
   5. Liquid culture of the host under selection pressure.
   6. Plasmid DNA isolation by the alkaline lysis method.
   7. Its restriction digestion with a common restriction endonuclease.
   8. Agarose gel electrophoresis against control.

3. PCR- An introduction to DNA-based diagnosis of human pathogens:
   1. Use a PCR-based kit for rapid diagnosis of Mycobacterium tuberculosis genome in human samples.
   2. Use synthetic control comprising of recombinant plasmid with Mycobacterium genome fragment cloned into it as positive control template DNA.
   3. Visualized the PCR amplicon profile on an agarose gel.
   4. Identifying synthetic positive and negative samples.
   OR

   PCR: Developing a Multiple Arbitrary Amplicon profile using Arbitrarily-Primed PCR:

   1. Use the method of PCR to generate a RAPD DNA fingerprint at low annealing temperature.
   2. Agarose gel electrophoresis of amplified fragments.
   3. Identifying different MAAP profile using different AP-PCR primer probes.

4. Identifying a Single Nucleotide Polymorphism (SNP) marker in the middle of a gene linked to production trait in cattle using the PCR-RFLP technology.
   1. Amplification of an exonic region of the gene from cattle genome.
   2. Confirming successful PCR amplification by agarose gel electrophoresis.
   3. Restriction digestion of the PCR product.
   4. Re checking on agarose gel for genotyping the animals.

5. Automated DNA Sequencing:
   1. Cycle sequencing of the PCR product using the Sanger’s chain termination method.
   2. Processing of the product for removal of unbound fluorescent nucleotides.
   3. Electrophoresis using an automated genetic analyzer and real time visualization of amplicon signals.
   4. Analyzing the sequence using off line as well as on line bioinformatics tools.

6. Real time PCR application:
   1. Amplification of a region of mammalian / bacterial genome using real time PCR.
   2. Tracking the PCR amplicons using SYBR Green fluorescence sensed by the laser detector of the machine.
   3. Undertaking a dissociation curve analysis to identify target and non-specific amplification as a function of its melting temperature and subsequent variation in fluorescence.